The multiplicity of the largest distance Laplacian eigenvalue in a connected graph. The distance Laplacian of a graph is the (symmetric) matrix with row and column sums $0$, which has the negative distances between two vertices as its off-diagonal entries. This statistic is the largest multiplicity of an eigenvalue. For example, the cycle on four vertices has distance Laplacian $$\left(\begin{array}{rrrr} 4 & -1 & -2 & -1 \\ -1 & 4 & -1 & -2 \\ -2 & -1 & 4 & -1 \\ -1 & -2 & -1 & 4 \end{array}\right).$$ Its eigenvalues are $0,4,4,6$, so the statistic is $1$. The path on four vertices has eigenvalues $0, 4.7\dots, 6, 9.2\dots$ and therefore also statistic $1$. The graphs with statistic $n-1$, $n-2$ and $n-3$ have been characterised, see [1].

The multiplicity of the largest Laplacian eigenvalue in a graph.

The distinguishing index of a graph. This is the smallest number of colours such that there is a colouring of the edges which is not preserved by any automorphism. If the graph has a connected component which is a single edge, or at least two isolated vertices, this statistic is undefined.

The largest eigenvalue of a graph if it is integral. If a graph is $d$-regular, then its largest eigenvalue equals $d$. One can show that the largest eigenvalue always lies between the average degree and the maximal degree. This statistic is undefined if the largest eigenvalue of the graph is not integral.

The girth of a graph, which is not a tree. This is the length of the shortest cycle in the graph.